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immunoelectron microscope fixative  (Servicebio Inc)

 
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    Servicebio Inc immunoelectron microscope fixative
    The interaction between pH108R protein and capsid proteins p49 and p72. <t>Immunoelectron</t> microscopy of PAMs infected with ASFV-GS (A) or ASFV-GS-ΔH108R (B). PAMs infected with ASFV-GS or ASFV-GS-ΔH108R were fixed and immunolabeled with a mouse anti-p72 monoclonal antibody followed by incubated with goat anti-mouse antibody conjugated to 12-nm gold particles. (C) Association of pH108R-flag with p49-myc. HEK293T cells were co-transfected with pH108R-flag and p49-myc constructs for 48 h, Co-immunoprecipitation (Co-IP) analysis was performed utilizing an anti-flag monoclonal antibody. (D) Interaction of pH108R with p49 during ASFV infection. Porcine alveolar macrophages (PAMs) were infected with ASFV-GS at an MOI of 1 for 36 h, Co-IP analysis was performed using an anti-p49 polyclonal antibody. (E) Immunofluorescence detection of pH108R and p49 in ASFV-infected cells. PAMs were infected with ASFV-GS at an MOI of 1 for 36 h, and then cells were fixed with 4% paraformaldehyde. Subsequently, fixed cells were labelled for pH108R (green) and p49 (red). Nucleus was stained with DAPI (blue). White arrows suggested co-localization at viral factories (VF). Scale bar, 10 μm. (F) Association of pH108R-flag with p72-myc. The method was the same as described in C. (G) Interaction of pH108R with p72 during ASFV infection. The method was same as described in D. (H) Immunofluorescence detection of pH108R and p72 in ASFV-infected cells. The method was the same as described in E. Scale bar, 10 μm.
    Immunoelectron Microscope Fixative, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/immunoelectron+microscope+fixative/pmc12903959-129-5-10?v=Servicebio+Inc
    Average 86 stars, based on 1 article reviews
    immunoelectron microscope fixative - by Bioz Stars, 2026-07
    86/100 stars

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    1) Product Images from "Involvement of the African swine fever virus protein pH108R in virion morphogenesis"

    Article Title: Involvement of the African swine fever virus protein pH108R in virion morphogenesis

    Journal: Emerging Microbes & Infections

    doi: 10.1080/22221751.2026.2620241

    The interaction between pH108R protein and capsid proteins p49 and p72. Immunoelectron microscopy of PAMs infected with ASFV-GS (A) or ASFV-GS-ΔH108R (B). PAMs infected with ASFV-GS or ASFV-GS-ΔH108R were fixed and immunolabeled with a mouse anti-p72 monoclonal antibody followed by incubated with goat anti-mouse antibody conjugated to 12-nm gold particles. (C) Association of pH108R-flag with p49-myc. HEK293T cells were co-transfected with pH108R-flag and p49-myc constructs for 48 h, Co-immunoprecipitation (Co-IP) analysis was performed utilizing an anti-flag monoclonal antibody. (D) Interaction of pH108R with p49 during ASFV infection. Porcine alveolar macrophages (PAMs) were infected with ASFV-GS at an MOI of 1 for 36 h, Co-IP analysis was performed using an anti-p49 polyclonal antibody. (E) Immunofluorescence detection of pH108R and p49 in ASFV-infected cells. PAMs were infected with ASFV-GS at an MOI of 1 for 36 h, and then cells were fixed with 4% paraformaldehyde. Subsequently, fixed cells were labelled for pH108R (green) and p49 (red). Nucleus was stained with DAPI (blue). White arrows suggested co-localization at viral factories (VF). Scale bar, 10 μm. (F) Association of pH108R-flag with p72-myc. The method was the same as described in C. (G) Interaction of pH108R with p72 during ASFV infection. The method was same as described in D. (H) Immunofluorescence detection of pH108R and p72 in ASFV-infected cells. The method was the same as described in E. Scale bar, 10 μm.
    Figure Legend Snippet: The interaction between pH108R protein and capsid proteins p49 and p72. Immunoelectron microscopy of PAMs infected with ASFV-GS (A) or ASFV-GS-ΔH108R (B). PAMs infected with ASFV-GS or ASFV-GS-ΔH108R were fixed and immunolabeled with a mouse anti-p72 monoclonal antibody followed by incubated with goat anti-mouse antibody conjugated to 12-nm gold particles. (C) Association of pH108R-flag with p49-myc. HEK293T cells were co-transfected with pH108R-flag and p49-myc constructs for 48 h, Co-immunoprecipitation (Co-IP) analysis was performed utilizing an anti-flag monoclonal antibody. (D) Interaction of pH108R with p49 during ASFV infection. Porcine alveolar macrophages (PAMs) were infected with ASFV-GS at an MOI of 1 for 36 h, Co-IP analysis was performed using an anti-p49 polyclonal antibody. (E) Immunofluorescence detection of pH108R and p49 in ASFV-infected cells. PAMs were infected with ASFV-GS at an MOI of 1 for 36 h, and then cells were fixed with 4% paraformaldehyde. Subsequently, fixed cells were labelled for pH108R (green) and p49 (red). Nucleus was stained with DAPI (blue). White arrows suggested co-localization at viral factories (VF). Scale bar, 10 μm. (F) Association of pH108R-flag with p72-myc. The method was the same as described in C. (G) Interaction of pH108R with p72 during ASFV infection. The method was same as described in D. (H) Immunofluorescence detection of pH108R and p72 in ASFV-infected cells. The method was the same as described in E. Scale bar, 10 μm.

    Techniques Used: Immuno-Electron Microscopy, Infection, Immunolabeling, Incubation, Transfection, Construct, Immunoprecipitation, Co-Immunoprecipitation Assay, Immunofluorescence, Staining



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    The interaction between pH108R protein and capsid proteins p49 and p72. <t>Immunoelectron</t> microscopy of PAMs infected with ASFV-GS (A) or ASFV-GS-ΔH108R (B). PAMs infected with ASFV-GS or ASFV-GS-ΔH108R were fixed and immunolabeled with a mouse anti-p72 monoclonal antibody followed by incubated with goat anti-mouse antibody conjugated to 12-nm gold particles. (C) Association of pH108R-flag with p49-myc. HEK293T cells were co-transfected with pH108R-flag and p49-myc constructs for 48 h, Co-immunoprecipitation (Co-IP) analysis was performed utilizing an anti-flag monoclonal antibody. (D) Interaction of pH108R with p49 during ASFV infection. Porcine alveolar macrophages (PAMs) were infected with ASFV-GS at an MOI of 1 for 36 h, Co-IP analysis was performed using an anti-p49 polyclonal antibody. (E) Immunofluorescence detection of pH108R and p49 in ASFV-infected cells. PAMs were infected with ASFV-GS at an MOI of 1 for 36 h, and then cells were fixed with 4% paraformaldehyde. Subsequently, fixed cells were labelled for pH108R (green) and p49 (red). Nucleus was stained with DAPI (blue). White arrows suggested co-localization at viral factories (VF). Scale bar, 10 μm. (F) Association of pH108R-flag with p72-myc. The method was the same as described in C. (G) Interaction of pH108R with p72 during ASFV infection. The method was same as described in D. (H) Immunofluorescence detection of pH108R and p72 in ASFV-infected cells. The method was the same as described in E. Scale bar, 10 μm.
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    The interaction between pH108R protein and capsid proteins p49 and p72. <t>Immunoelectron</t> microscopy of PAMs infected with ASFV-GS (A) or ASFV-GS-ΔH108R (B). PAMs infected with ASFV-GS or ASFV-GS-ΔH108R were fixed and immunolabeled with a mouse anti-p72 monoclonal antibody followed by incubated with goat anti-mouse antibody conjugated to 12-nm gold particles. (C) Association of pH108R-flag with p49-myc. HEK293T cells were co-transfected with pH108R-flag and p49-myc constructs for 48 h, Co-immunoprecipitation (Co-IP) analysis was performed utilizing an anti-flag monoclonal antibody. (D) Interaction of pH108R with p49 during ASFV infection. Porcine alveolar macrophages (PAMs) were infected with ASFV-GS at an MOI of 1 for 36 h, Co-IP analysis was performed using an anti-p49 polyclonal antibody. (E) Immunofluorescence detection of pH108R and p49 in ASFV-infected cells. PAMs were infected with ASFV-GS at an MOI of 1 for 36 h, and then cells were fixed with 4% paraformaldehyde. Subsequently, fixed cells were labelled for pH108R (green) and p49 (red). Nucleus was stained with DAPI (blue). White arrows suggested co-localization at viral factories (VF). Scale bar, 10 μm. (F) Association of pH108R-flag with p72-myc. The method was the same as described in C. (G) Interaction of pH108R with p72 during ASFV infection. The method was same as described in D. (H) Immunofluorescence detection of pH108R and p72 in ASFV-infected cells. The method was the same as described in E. Scale bar, 10 μm.
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    The interaction between pH108R protein and capsid proteins p49 and p72. Immunoelectron microscopy of PAMs infected with ASFV-GS (A) or ASFV-GS-ΔH108R (B). PAMs infected with ASFV-GS or ASFV-GS-ΔH108R were fixed and immunolabeled with a mouse anti-p72 monoclonal antibody followed by incubated with goat anti-mouse antibody conjugated to 12-nm gold particles. (C) Association of pH108R-flag with p49-myc. HEK293T cells were co-transfected with pH108R-flag and p49-myc constructs for 48 h, Co-immunoprecipitation (Co-IP) analysis was performed utilizing an anti-flag monoclonal antibody. (D) Interaction of pH108R with p49 during ASFV infection. Porcine alveolar macrophages (PAMs) were infected with ASFV-GS at an MOI of 1 for 36 h, Co-IP analysis was performed using an anti-p49 polyclonal antibody. (E) Immunofluorescence detection of pH108R and p49 in ASFV-infected cells. PAMs were infected with ASFV-GS at an MOI of 1 for 36 h, and then cells were fixed with 4% paraformaldehyde. Subsequently, fixed cells were labelled for pH108R (green) and p49 (red). Nucleus was stained with DAPI (blue). White arrows suggested co-localization at viral factories (VF). Scale bar, 10 μm. (F) Association of pH108R-flag with p72-myc. The method was the same as described in C. (G) Interaction of pH108R with p72 during ASFV infection. The method was same as described in D. (H) Immunofluorescence detection of pH108R and p72 in ASFV-infected cells. The method was the same as described in E. Scale bar, 10 μm.

    Journal: Emerging Microbes & Infections

    Article Title: Involvement of the African swine fever virus protein pH108R in virion morphogenesis

    doi: 10.1080/22221751.2026.2620241

    Figure Lengend Snippet: The interaction between pH108R protein and capsid proteins p49 and p72. Immunoelectron microscopy of PAMs infected with ASFV-GS (A) or ASFV-GS-ΔH108R (B). PAMs infected with ASFV-GS or ASFV-GS-ΔH108R were fixed and immunolabeled with a mouse anti-p72 monoclonal antibody followed by incubated with goat anti-mouse antibody conjugated to 12-nm gold particles. (C) Association of pH108R-flag with p49-myc. HEK293T cells were co-transfected with pH108R-flag and p49-myc constructs for 48 h, Co-immunoprecipitation (Co-IP) analysis was performed utilizing an anti-flag monoclonal antibody. (D) Interaction of pH108R with p49 during ASFV infection. Porcine alveolar macrophages (PAMs) were infected with ASFV-GS at an MOI of 1 for 36 h, Co-IP analysis was performed using an anti-p49 polyclonal antibody. (E) Immunofluorescence detection of pH108R and p49 in ASFV-infected cells. PAMs were infected with ASFV-GS at an MOI of 1 for 36 h, and then cells were fixed with 4% paraformaldehyde. Subsequently, fixed cells were labelled for pH108R (green) and p49 (red). Nucleus was stained with DAPI (blue). White arrows suggested co-localization at viral factories (VF). Scale bar, 10 μm. (F) Association of pH108R-flag with p72-myc. The method was the same as described in C. (G) Interaction of pH108R with p72 during ASFV infection. The method was same as described in D. (H) Immunofluorescence detection of pH108R and p72 in ASFV-infected cells. The method was the same as described in E. Scale bar, 10 μm.

    Article Snippet: Cells were further fixed with immunoelectron microscope fixative (Catalogue #G1124-100ML, Servicebio, Wuhan, China) for 2 h at 4°C.

    Techniques: Immuno-Electron Microscopy, Infection, Immunolabeling, Incubation, Transfection, Construct, Immunoprecipitation, Co-Immunoprecipitation Assay, Immunofluorescence, Staining